Biopsy is essential for the assessment of aggressive-looking bone and soft tissue tumors if accurate diagnosis is not possible by clinical and radiological examinations. Biopsy of the suspected malignant lesions should be performed in the main treatment centers. However, for the right to guide of the biopsy, the clinical and radiological data should be evaluated by a multidisciplinary council. The technique and localization of the biopsy must be well planned, and pathology physician should be informed at each step. A successful biopsy is closely related to the type of lesion, physical facilities and the expertise of the physician.