Two-stage surgery is considered as the gold standard treatment in chronic periprosthetic joint infections. Following the removal of the infected implant during the first phase, spacers are used which release local antibiotics in addition to systemic antibiotics. Spacers are useful in treatment of infection by releasing antibiotics as well as keeping the tension of the soft tissues until the second stage. There are many different applications of spacers. The prefabricated or the hand made ones, prepared during the surgery; cement mixed with antibiotics and selection of antibiotics; dynamic or static ones are different options. In this paper, various applications of spacers and antibiotic loaded cements are reviewed.