Soft tissue sarcomas (STS) are extremely rare malignant tumors arising from mesenchymal tissue in an entire region of the body. Clinical, radiological and histopathological multidisciplinary approach, diagnosis, treatment and follow-up team are extremely important. Although sometimes the diagnosis can be made only by careful history, physical examination and imaging methods, the final diagnosis is made by an experienced pathologist examining the pathological results obtained in sufficient quantities and from the right place. Accurate diagnosis is extremely important in terms of staging the disease and planning the treatment. Biopsy is a very important and complex surgical procedure applied in the staging process. In order not to adversely affect the result, the next steps must be carefully planned and implemented. Obtaining sufficient tissue samples for diagnosis without spreading the tumor, following the surgical technique rules, should be the main goal. Biopsy performed with the right technique plays a key role in the diagnosis and treatment phase. Cor needle, incisional and excisional biopsy techniques are frequently applied. The selection of the appropriate biopsy technique depends on the size and location of the lesion, and the experience of the surgeon and pathologist. Correct technique and duly performed soft tissue biopsy allows sufficient diagnostic material to be obtained and facilitates the pathologist`s work in the diagnosis process.