Pain originating from joint problems, and spinal or tendon disorders is one of the commonly seen musculoskeletal problems. Prolotherapy, which is a supportive treatment and applied intraarticularly, has gained popularity in recent years. Proliferative or sclerosing solutions, such as hypertonic dextrose, are used in the prolotherapy method which is suggested to have a pain relief effect and enhance functional capacity. Injections made specifically to tendon-bone junctions or ligament-bone attachment sites are intended to activate the body`s healing pathways and trigger inflammation. There are studies reporting that the prolotherapy technique applied by trained specialists is effective in reducing the pain and in improving the functional outcome. Although there is no `Level 1` study yet to show that prolotherapy is effective on clinical outcomes, reports of recent studies have value as pioneering works.