In accordance with other medical branches, regenerative medicine applications as tissue engineering and cell therapies have found a wide range of scientific research opportunities and grows rapidly in the musculoskeletal field. The search for the ideal source and donor site of these treatments is still going on. Treatment and tissue engineering with amniotic membrane and amniotic fluid derived stem cells emerges as a new potential in regenerative medicine and the treatment of musculoskeletal system, with the advantages of non-invasive isolation, multipotency, rapid self regeneration, low immunogenecity, anti-inflammatory effect, non-tumorigenic feature, and limited ethical problems.