Human articular cartilage is fed via the synovial fluid and the bone; therefore, its healing ability is limited. Palliative procedures such as debridement, microfracture, and drilling in cartilage lesions result in the formation of fibrous cartilage instead of hyaline cartilage. Autologous chondrocyte implantation and matrix-induced autologous chondrocyte implantation have been commonly applied as the treatment of cartilage lesions in recent years. The main objective of these techniques is to obtain a healing tissue closest to the hyaline cartilage in the means of regenerating tissue. In this paper, information about the indications, contraindications, application techniques, complications, postoperative rehabilitation, advantages and disadvantages of two-stage autologous chondrocyte implantation were reviewed.