Mesenchymal stem cells have been started to be used widely as the treatment of musculoskeletal disorders in recent years. As a source of mesenchymal stem cells, adipose tissue has begun to be used more and more in addition to bone marrow. Adipose tissue derived stem cells may be applied as cultivated but also as stromal vascular fraction which is comprised of different cells and extracellular matrix. Since stromal vascular fraction (SVF) is fast derived, cheap, practical, capable to differentiate various tissues it bears capability to be used for bone, muscle and soft tissue damages. Stromal vascular fraction can be derived from adipose tissue by enzimatic or mechanical processes. There are studies which support the effectiveness of SVF on tendon and bone tissue healing and cartilage regeneration by means of its stem cell and extracellular matrix ingredients. However, to prove the effectiveness as treatment studies with high evidence level and long term follow up are needed.